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A Study on the Inhibitory Effect and Mechanism of Lonicera Japonica on Type I Interferon
A Study on the Inhibitory Effect and Mechanism of Lonicera Japonica on Type I Interferon
The Journal of Korean Obstetrics and Gynecology. 2013. May, 26(2): 17-32
  • Published : May 31, 2013
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Kang, Yong-Goo
Ryu, Ik-Han
Kim, Song-Baek
Choi, Chang-Min
Seo, Yun-Jung
Cho, Han-Baek

Abstract
Objectives: The purpose of this study was to investigate whether Lonicera japonica(LJ) could inhibit LPS-induced type I IFN production. Methods: To evaluate inhibitory effect of LJ on type I IFN, we examined type I IFN, IRF-1, 7 and IL-10 production on LPS-induced macrophages using real time RT-PCR. Next, we observed the interaction of type I IFN, IRF-1, 7 and IL-10 using IL-10 neutralizing antibody. Finally we examined the activation of STAT-1, 3 using western blot. Results: LJ inhibited Type I IFN expression of mRNA and increased IL-10 expression of mRNA. Also LJ inhibited the level of IRF-1, 7 mRNA and the nuclear translocation of IRF-3. Further more, LJ reduced the activation of STAT-1, 3 which are involved in continuous secretion of immune cytokines. Blockade of IL-10 action caused a significant reduction of type I IFN and IRF-1, 7 than LPS-induced LJ pretreatment. Conclusions: LJ inhibits LPS-induced production of type I IFN by IL-10. This study may provide a clinical basis for anti-inflammatory properties of LJ.
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